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KMID : 0353320130370020082
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Oral Biology Research
2013 Volume.37 No. 2 p.82 ~ p.87
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Transcriptional expression of arg and art genes in pst pit mutants of Salmonella Typhimurium according to extracellular inorganic phosphate levels
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Park Yoon-Mee
Bang Iel-Soo
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Abstract
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Purpose: Salmonella enterica serovar Typhimurium (S. Typhimurium) can cause salmonellosis, including gastroenteritis and systemic infection in animal hosts such as humans by diet contamination. To replicate and survive in an animal host, S. Typhimurium must compete against host cells for nutrients. Inorganic phosphate (Pi) is an essential element for all organisms and is used for cellular signal transduction and synthesis of various biomolecules. Thus, systems for uptake and homeostasis of Pi are required for cell metabolism. Uptake of Pi in S. Typhimurium is mainly regulated by components of the high-affinity adenosine triphosphate-binding cassette-transport system Pst and low-affinity transporter Pit. To identify genetic factors contributing to homeostasis of Pi, this study investigated the transcriptional profiles of Salmonella mutants lacking Pst and Pit transporters experiencing changes in extracellular Pi.
Materials and Methods: Transcriptome of pst pit mutant S. Typhimurium in response to low (0.1 mM) and high (10 mM) extracellular Pi were compared by cDNA microarray analysis, respectively.
Results: The results show that transcription of argI encoding ornithine carbamoyltransferase and artJ encoding arginine third transport system was significantly induced.
Conclusion: Data suggest a relationship between arginine metabolism and Pi homeostasis in S. Typhimurium.
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KEYWORD
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Phosphate transporter proteins, Phosphate-specific transport protein system, Arginine, Salmonella
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